PDB ID Mutant Temp Kd ∆G ∆∆G PMID 1FEU_A D 87E 273 2.50E-06 -6.69 1.73 15718233 1FEU_A H 85A 273 1.20E-06 -7.07 1.35 15718233 1FEU_A K 14A 273 1.30E-07 -8.22 0.2 15718233 1FEU_A R 10A 273 1.40E-06 -6.99 1.43 15718233 1FEU_A R 19A 273 1.25E-06 -7.05 1.37 15718233 1FEU_A R 20A 273 1.50E-07 -8.15 0.15 15718233 1FEU_A S 16A 273 1.20E-07 -8.27 0.15 15718233 1WNE_A K 20E 310 7.60E-05 -5.59 0.32 25903341 1ZDI_A K 43A 277 2.50E-09 -10.42 -0.09 16380130 1ZDI_A K 57A 277 3.30E-07 -7.85 2.48 16380130 1ZDI_A K 61A 277 3.00E-07 -7.9 2.43 16380130 1ZDI_A R 49A 277 7.07E-08 -8.67 1.66 16380130 1ZDI_A S 52A 277 1.60E-09 -10.66 -0.33 16380130 1ZDI_A Y 85A 277 2.00E-06 -6.91 3.42 16380130 2KXN_B I 195A 313 1.23E-05 -6.72 1.01 21399644 2KXN_B P 199A 313 7.10E-06 -7.05 0.68 21399644 2KXN_B R 111A 313 3.65E-06 -7.45 0.29 21399644 2KXN_B S 194A 313 3.25E-06 -7.52 0.22 21399644 2KXN_B T 196A 313 1.50E-05 -6.61 1.13 21399644 2XB2_G R 423A 295 1.20E-04 -5.06 2.58 20479275 2XB2_G Y 429A 295 1.00E-03 -3.87 3.77 20479275 3AM1_A K 207A 273 1.10E-08 -9.51 -0.66 20870747 3UZS_A H 280A 277 2.11E-07 -8.09 0.44 22727813 3VYY_A N 30A 298 1.45E-05 -6.31 0.5 23361462 4CIO_A A 110T 298 5.65E-07 -8.15 1.19 25183497 4CIO_A G 113E 298 2.46E-07 -8.62 0.72 25183497 4CIO_A K 36E 298 3.34E-06 -7.14 2.2 25183497 4CIO_A K 36M 298 6.80E-06 -6.74 2.6 25183497 4CIO_A K 74M 298 4.80E-08 -9.54 -0.2 25183497 4CIO_A N 106A 298 1.58E-07 -8.87 0.47 25183497 4CIO_A N 108A 298 5.10E-08 -9.51 -0.17 25183497 4CIO_A R 103M 298 2.99E-07 -8.51 0.83 25183497 4CIO_A R 76M 298 3.50E-08 -9.72 -0.38 25183497 4CIO_A Y 44A 298 1.06E-06 -7.79 1.35 25183497 4CIO_A Y 44F 298 9.80E-08 -9.14 0.2 25183497 4CIO_A Y 78F 298 8.55E-07 -7.91 1.43 25183497 4G0A_A K 223A 293 3.54E-07 -8.27 1.28 22811529 4JVH_A K 120A 298 1.90E-06 -7.46 1.87 23630077 4JVH_A K 190A 298 8.90E-06 -6.58 2.75 23630077 4JVH_A N 97A 298 1.00E-06 -7.82 1.51 23630077 4JVH_A Q 193A 298 8.90E-06 -6.58 2.75 23630077 4JVH_A R 124A 298 1.90E-06 -7.46 1.87 23630077 4JVH_A R 130 A 298 1.00E-06 -7.82 1.51 23630077 4NL3_D F 43W 298 1.14E-08 -10.32 0.74 25150227 4NL3_D Q 6A 298 5.00E-07 -8.16 2.9 25150227 5EN1_A D 49A 298 5.62E-07 -8.15 0.9 Wu, Baixing, et al. bioRxiv (2017) 5EN1_A F 24A 298 4.57E-07 -8.27 0.78 Wu, Baixing, et al. bioRxiv (2017) 5EN1_A F 66A 298 7.63E-07 -7.98 1.07 Wu, Baixing, et al. bioRxiv (2017) 5EN1_A H 108A 298 1.67E-07 -8.84 0.21 Wu, Baixing, et al. bioRxiv (2017) 5EV1_A Q 147A 296 1.71E-07 -8.76 -0.32 26952537 5EV1_A R 227A 296 1.66E-07 -8.78 -0.33 26952537 5EV1_A V 254P 296 1.37E-07 -8.89 -0.44 26952537 5HO4_A D 49A 298 5.62E-07 -8.15 0.9 Wu, Baixing, et al. bioRxiv (2017) 5HO4_A E 92A 298 7.52E-07 -7.98 1.06 Wu, Baixing, et al. bioRxiv (2017) 5HO4_A F 24A 298 4.57E-07 -8.27 0.78 Wu, Baixing, et al. bioRxiv (2017) 5HO4_A F 66A 298 7.63E-07 -7.98 1.07 Wu, Baixing, et al. bioRxiv (2017) 5HO4_A H 108A 298 1.67E-07 -8.84 0.21 Wu, Baixing, et al. bioRxiv (2017) 5HO4_A Q 19A 298 4.41E-07 -8.29 0.76 Wu, Baixing, et al. bioRxiv (2017)